Description
Principle of the Assay: An anti-TSH coating antibody is adsorbed onto a microtiter plate. TSH protein present in the sample or standard binds to the antibodies adsorbed on the plate; a biotinylated anti-TSH antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a streptavidin-enzyme conjugate is added and binds to the biotinylated anti-TSH antibody. Unbound streptavidin-enzyme conjugate is removed during a wash step, and substrate solution is added to the wells. A colored product is formed in proportion to the amount of TSH present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from purified TSH and sample concentration is then determined: